control aav aav8 vil null constructs  (Shanghai Genechem Ltd)

Journal: Metabolism Open

Article Title: Hepatic activating transcription factor 3 protects against systemic inflammation by attenuating lipotoxicity

doi: 10.1016/j.metop.2025.100417

Figure Lengend Snippet: Overexpession of hepatic ATF3 attenuates high-fat diet-induced systemic inflammatory responses. C 5 7BL /6J mice were injected with AAV8-TBG-Null or AAV8-TBG-hATF3 via the tail vein, then fed a high fat diet for 16 weeks (n = 6 per group). (A) Hepatic protein levels were determined by Western blot assays. (B–D) Plasma proinflammatory cytokines levels were analyzed. Inflammation-related mRNA levels were determined in the liver (E), inguinal white adipose tissue (iWAT) (F), and epididymal white adipose tissue (eWAT) (G). All values are expressed as mean ± SEM. ∗ P < 0.05 vs. control.

Article Snippet: AAV8-TBG-Null (control), AAV8-TBG-Cre, and AAV8-TBG -hATF3 were produced and titrated by OBiO Technology Corp (Shanghai).

Techniques: Injection, Western Blot, Clinical Proteomics, Control

Journal: Metabolism Open

Article Title: Hepatic activating transcription factor 3 protects against systemic inflammation by attenuating lipotoxicity

doi: 10.1016/j.metop.2025.100417

Figure Lengend Snippet: High expression of human ATF3 in hepatocytes reverses systemic inflammatory responses in db/db mice. Db/db mice were injected with AAV8-TBG-Null or AAV8-TBG-hATF3 via the tail vein (n = 6 per group). (A) Hepatic protein levels were determined by Western blot assays. (B–D) Plasma proinflammatory cytokines levels were analyzed. Inflammation-related mRNA levels were determined in iWAT (E), and eWAT (F). All values are expressed as mean ± SEM. ∗ P < 0.05 vs. control.

Article Snippet: AAV8-TBG-Null (control), AAV8-TBG-Cre, and AAV8-TBG -hATF3 were produced and titrated by OBiO Technology Corp (Shanghai).

Techniques: Expressing, Injection, Western Blot, Clinical Proteomics, Control

Journal: Metabolism Open

Article Title: Hepatic activating transcription factor 3 protects against systemic inflammation by attenuating lipotoxicity

doi: 10.1016/j.metop.2025.100417

Figure Lengend Snippet: Hepatocytic ATF3 is required for protection against HF diet-induced hepatic lipotoxicity likely mediated through AMPKα activation. (A–F) C57BL/6J mice were injected with AAV8-TBG-Null or AAV8-TBG-hATF3 via the tail vein, then fed an HF diet for 16 weeks (n = 6 per group). Hepatic levels of triglyceride (A), total cholesterol (B) and free fat acid (C) were quantified. (D) Hepatic reactive oxygen species levels. (E) Oil red O staining of liver sections. (F) Hepatic protein levels were determined by Western blot assays and quantified. (G–L) Atf3 fl/fl and Atf3 Hep−/− mice were fed an HF diet for 20 weeks (n = 6 per group). Hepatic levels of triglyceride (G), total cholesterol (H) and free fat acid (I) were quantified. (J) Hepatic reactive oxygen species levels. (K) Oil red O staining of liver sections. (L) Hepatic protein levels were determined by Western blot assays and quantified. Scale bars in E and K: 50 μm. All values are expressed as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01 vs. control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: AAV8-TBG-Null (control), AAV8-TBG-Cre, and AAV8-TBG -hATF3 were produced and titrated by OBiO Technology Corp (Shanghai).

Techniques: Activation Assay, Injection, Staining, Western Blot, Control

Journal: Metabolism Open

Article Title: Hepatic activating transcription factor 3 protects against systemic inflammation by attenuating lipotoxicity

doi: 10.1016/j.metop.2025.100417

Figure Lengend Snippet: Hepatocyte ATF3 attenuates FFAs-mediated proinflammatory effects in adipose tissue explants. (A–C) Primary hepatocytes were isolated from mice injected with AAV8-TBG-Null or AAV8-TBG-hATF3, which were incubated with lipid mixture containing FFAs for 24 h. The culture media were collected and then used to treat adipose tissue explants for an additional 24 h (n = 6 per group). (B) Proinflammatory cytokines levels in media were analyzed. (C) Inflammation-related mRNA levels in adipose tissue explants were determined. (D and E) Primary hepatocytes were isolated from Atf3 fl/fl and Atf3 Hep−/− mice and subsequently processed using the same method as described above. (D) Proinflammatory cytokines levels in media were analyzed. (E) Inflammation-related mRNA levels in adipose tissue explants were determined. Primary hepatocytes were isolated from mice injected with AAV8-TBG-Null or AAV8-TBG-hATF3 (F), or Aft3 fl/fl and Atf3 Hep−/− mice (G). After 24 h, the culture media were collected and used to treat adipose tissue explants for another 24 h. (F and G) Inflammation-related mRNA levels in adipose tissue explants were determined. All values are expressed as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01 vs. control.

Article Snippet: AAV8-TBG-Null (control), AAV8-TBG-Cre, and AAV8-TBG -hATF3 were produced and titrated by OBiO Technology Corp (Shanghai).

Techniques: Isolation, Injection, Incubation, Control